704 DEVELOPMENT OF GLUTAMINASE ALONG THE VILLUS-CRYPT AXIS OF RAT JEJUNUM
نویسندگان
چکیده
منابع مشابه
Distribution and dietary induction of cellular retinol-binding protein type II along the villus-crypt axis of the rat jejunum.
Cellular retinol-binding protein type II (CRBPII) is exclusively expressed in the small intestinal absorptive cells. We previously reported that dietary fat induces CRBPII expression within 12 h of fat intake. To examine at which locus of the villus-crypt axis this response to dietary fat occurs, 6-wk-old rats were fed a low-fat diet (7% energy) for 7 d, and then given free access to a high-fat...
متن کاملRegulation of sucrase-isomaltase gene expression along the crypt-villus axis of rat small intestine.
The expression of sucrase-isomaltase mRNA was investigated along the crypt-villus axis of rat small intestine using differentially isolated cells and in situ hybridization. A partial rat sucrase-isomaltase cDNA was cloned which coded for a protein that was predicted to be 88% homologous to those encoded by the rabbit and human cDNAs. Southern blot analysis of rat genomic DNA indicated that the ...
متن کاملDifferential proteome analysis along jejunal crypt-villus axis in piglets.
The brush-border membrane (BBM) of enterocytes is responsible for the digestion and absorption of nutrients and ions in the small intestine. To identify the BBM proteins involved in epithelial cell maturation along the crypt-villus axis, enterocytes were sequentially isolated from the villus tip to the crypt of the jejunum from 21-day-old suckling piglets. After preparation of BBM vesicles, we ...
متن کاملDevelopmental pattern of rat intestinal brush-border enzymic proteins along the villus--crypt axis.
At various postnatal stages, intestinal epithelial cells were isolated sequentially from villus tip to crypt base by successive EDTA treatments. According to the localization of marker enzymic activities, isolated cells were pooled into three cell compartments: villus (V), lower villus and upper crypt (VC) and crypt (C). Purified brush-border-membrane proteins were separated by 7.5%-polyacrylam...
متن کاملChanges of immunoreactive sucrase-isomaltase complex in rat small intestine during postnatal development and maturation along the villus-crypt axis.
Appearance of immunoreactive sucrase-isomaltase complex was observed in rat small intestine during postnatal development and maturation along the villus-crypt axis by single radial immunodiffusion. The immunoreactive sucrase-isomaltase complex in brush-border mem brane increased in parallel with enzyme activities until weaning. After weaning, higher amounts of the immunoreactive enzyme proteins...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Pediatric Research
سال: 1985
ISSN: 0031-3998,1530-0447
DOI: 10.1203/00006450-198504000-00734